FCS and FRAP: illuminating cellular processes
نویسندگان
چکیده
منابع مشابه
On the Equivalence of Fcs and Frap: Simultaneous Lipid Membrane Measurements by Imaging Fcs
Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Recovery after Photobleaching (FRAP) are probably the most widely used methods to determine diffusion coefficients in biological samples. However, it has already been noted in the past that the two methods do not necessarily provide the same results. This has typically been explained by the different spatial range over which the two m...
متن کاملOn the Equivalence of FCS and FRAP: Simultaneous Lipid Membrane Measurements.
Fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRAP) are widely used methods to determine diffusion coefficients. However, they often do not yield the same results. With the advent of camera-based imaging FCS, which measures the diffusion coefficient in each pixel of an image, and proper bleaching corrections, it is now possible to measure the diffus...
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ژورنال
عنوان ژورنال: Nature Cell Biology
سال: 2009
ISSN: 1465-7392,1476-4679
DOI: 10.1038/ncb1945